FAQs

Frequently Asked Questions

 
The Basics

Q: How does the Cellix system work?
A: The Cellix platform provides researchers in pharmaceutical and biotech companies with a groundbreaking new solution to enhance their drug discovery programmes and eliminate false leads earlier. Cellix's unique technology enables cell-based flow assays in a relevant physiological microenvironment mimicking blood capillaries. Cell-cell and cell-protein interactions can be studied in a fast, reliable, cost-effective manner.
 
Q: What stage in the drug screening process is the Cellix system used?
A: The Cellix platform is used to optimise leads before engaging in animal trials. It simulates blood capillaries so human isolated cells or whole blood can be used for flow assays, marking an important step forward from in vitro static experiments. This means more accurate results can be achieved before proceeding to animal models and clinical trials saving time and money spent on false leads. 

The Benefits

Q: What are the advantages of the Cellix VenaFlux platform over other technologies/techniques?
A: Our clients have reported that the Cellix platform provides information much faster, resulting in a reduction in experimental time and overall costs. The VenaFlux is a fully integrated, automatic platform that enables researchers to carry out experiments in conditions that mimic physiological microenvironments. Cell-based flow assays with a wide range of shear stresses compete with the standard static experiments that are currently used in laboratories
 
Q: I am currently using my own customised system, what can Cellix do to improve my drug screening process?
A: Cellix's technology can be integrated and adapted to work with many in-house systems to enhance the precision and accuracy of your drug screening process.
 
Q: I am not currently using anything like this, how will using Cellix technology impact my drug screening process?
A: Cellix's technology can enhance the drug screening process by simulating the flow of cells in blood capillaries. Compounds can be investigated with isolated cells or whole blood, providing more accurate results; before proceeding to animal models and clinical trials saving time and money spent on false leads.
 
Q: Because Cellix adds another stage into my drug screening process won't it slow down how quickly I can optimise my leads?
A: As another step is added to the discovery process, savings in time and expenses are reflected in the quality of results obtained. Relatively rapid analyses reduces drug development costs by accelerating false lead elimination and increasing productivity in pharmaceutical and biotechnology research and development laboratories. 

Product Specific Questions

Q: What type of information does the VenaFlux platform provide?
A: The adhesion profiles of cells can be studied to obtain information, for example, on the effects of drugs, on receptor-ligand activation, on the effects of chemokines in research areas such as cardiovascular, oncology, respiratory or bacteriology.
 
Q: Does the user require certain knowledge or long training?
A: During the installation of the platform in your laboratory all information and training will be provided. The platform is very straightforward and the software for running experiments and analysing results is extremely user-friendly.
 
Q: What type of experiments can be carried out?
A: Routinely experiments are carried out by Cellix's R&D team to study the adhesion profiles of cells such as monocytes, T-cells, eosinophils, neutrophils, PBMCs, cancer cells, platelets and cell lines including THP1s and CHOs. Studies with whole blood can also be performed. The adhesion of cells can be studied on endothelial cells or purified molecules such as rhVCAM-1, rhICAM-1, collagen, vWF or fibrinogen, depending on your application. If you would like to discuss whether Cellix's platform can be used for your scientific interests please contact us at mycells@tebu-bio.com and our team will assist you.
 
Q: What are the typical values of shear stress for the experiments?
A: Shear stress values change considerably when you're carrying out experiments using cell suspensions or whole blood. Typical values may vary from 0.1 to 20 dyne/cm2 (10-2000 s-1) for cell suspension, or from 30 to 180 dyne/cm2 (667-4000 s-1) for whole blood experiments, with the aim of reproducing physiological conditions or mimicking thrombosis.
 
Q: What objectives are typically used with the Vena8 Fluoro+?
A: Vena8 Fluoro+ Biochips contain 8 parallel enclosed microcapillaries for continuous flow cell based assays. Each microcapillary may be coated with a different adhesion molecule. Cell suspensions may then be injected using the MirusTM Nanopump which supports a range of shear stresses for dynamic flow based assays.

Q: What objectives are typically used with the Vena8 Endothelial+?
A:Vena8 Endothelial+TM Biochips contain 8 parallel enclosed microcapillaries for culturing primary endothelial cells and continuous flow cell based assays. Primary endothelial cells are cultured and cell suspensions may then be injected.
 
Q: How are the results analysed?
A: DucoCell is the software package supplied by Cellix for the image analysis. It provides the researcher with useful information, for example on cell number, cell shape, polarity, form-factor and area. Graphs are automatically generated for the selected parameters.

Therapeutic Areas

Q: What therapeutic areas has Cellix been validated in?
A: In collaboration with different partners (university groups and industry) Cellix's platform has been validated for a number of therapeutics areas including cardiovascular, oncology, respiratory and bacteriology. Many studies are on-going.  For more information about studies we are currently working on, please ask our team at mycells@tebu-bio.com.
 
Q: I want to use this for an area which currently hasn't been validated yet, what should I do?
A: If you don't know if Cellix's platform suits your scientific needs or matches your interests, you can contact our R&D team and propose your enquiries. We regularly conduct experiments in-house as requested by various organisations, to determine if a particular application is suitable to Cellix's technology. Alternatively, an on-site demonstration could be arranged to validate your particular application.